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rt pcr kit  (Bio-Rad)


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    Structured Review

    Bio-Rad rt pcr kit
    Rt Pcr Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 6819 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iscript+rt-pcr+kit/IScript+C+DNA+Synthesis+Kit/pmc12906651-65-11-13
    Average 99 stars, based on 6819 article reviews
    rt pcr kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Quantitative RT-PCR:

    Article Title: Modulation of TLR4/NFκB Pathways in Autoimmune Myocarditis
    Article Snippet: An iScript RT-PCR kit (Bio-Rad, Hercules, CA, USA) was used to synthesize the first-strand cDNA [ ].

    Article Title: Enhanced Survival of Plasmodium -Infected Mosquitoes during Starvation
    Article Snippet: Total RNA was extracted from individual mosquito using the RNeasy Mini Kit (QIAGEN, CA). cDNA was synthesized using the iScript RT-PCR kit (Bio-Rad, CA).

    Article Title: Compositions and methods for dengue virus chimeric constructions in vaccines
    Article Snippet: The real time RT-PCR was performed with the iQ5 or CFX-95 system (BioRad), using a BioRad iScript RT-PCR (for probes) kit, in a 25-μl reaction containing 5 μl of viral RNA template, 0.4 uM of each primer, and 0.2 uM of the probe.

    Article Title: Identification and Characterization of Ixodes scapularis Antigens That Elicit Tick Immunity Using Yeast Surface Display
    Article Snippet: The same procedure was done with unfed ticks. cDNA was synthesized using the iScript RT-PCR kit (Biorad, CA) and analyzed by quantitative PCR for the expression of tick actin, p8, p19, p23 and p32 and genes using the primers listed in .

    Article Title: NOTCH2 Promotes Osteoclast Maturation and Metabolism and Modulates the Transcriptome Profile During Osteoclastogenesis.
    Article Snippet: Equal amounts of RNA were reverse-transcribed using the iScript RT-PCR kit (Bio-Rad, Hercules, CA) and amplified in the presence of specific primers (all from IDT) with the SsoAdvancedTM Universal SYBR Green Supermix (Bio-Rad) at 60oC for 40 cycles (Table S6).

    Article Title: COLD REGULATED GENE 27 and 28 antagonize the transcriptional activity of the RVE8/LNK1/LNK2 circadian complex
    Article Snippet: One μ g of total RNA was used as the template to synthesize cDNA using the iScript RT-PCR kit (Bio-Rad, Carlsbad, CA).

    Article Title: Effect of Pesticide Vinclozolin Toxicity Exposure on Cardiac Oxidative Stress and Myocardial Damage
    Article Snippet: An iScript RT-PCR kit (Bio-Rad, Hercules, CA, USA) was used to synthesize first-strand cDNA according to the manufacturer’s recommendations.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Modulation of TLR4/NFκB Pathways in Autoimmune Myocarditis
    Article Snippet: An iScript RT-PCR kit (Bio-Rad, Hercules, CA, USA) was used to synthesize the first-strand cDNA [ ].

    Article Title: Enhanced Survival of Plasmodium -Infected Mosquitoes during Starvation
    Article Snippet: Total RNA was extracted from individual mosquito using the RNeasy Mini Kit (QIAGEN, CA). cDNA was synthesized using the iScript RT-PCR kit (Bio-Rad, CA).

    Article Title: Compositions and methods for dengue virus chimeric constructions in vaccines
    Article Snippet: The real time RT-PCR was performed with the iQ5 or CFX-95 system (BioRad), using a BioRad iScript RT-PCR (for probes) kit, in a 25-μl reaction containing 5 μl of viral RNA template, 0.4 uM of each primer, and 0.2 uM of the probe.

    Article Title: Identification and Characterization of Ixodes scapularis Antigens That Elicit Tick Immunity Using Yeast Surface Display
    Article Snippet: The same procedure was done with unfed ticks. cDNA was synthesized using the iScript RT-PCR kit (Biorad, CA) and analyzed by quantitative PCR for the expression of tick actin, p8, p19, p23 and p32 and genes using the primers listed in .

    Article Title: NOTCH2 Promotes Osteoclast Maturation and Metabolism and Modulates the Transcriptome Profile During Osteoclastogenesis.
    Article Snippet: Equal amounts of RNA were reverse-transcribed using the iScript RT-PCR kit (Bio-Rad, Hercules, CA) and amplified in the presence of specific primers (all from IDT) with the SsoAdvancedTM Universal SYBR Green Supermix (Bio-Rad) at 60oC for 40 cycles (Table S6).

    Article Title: COLD REGULATED GENE 27 and 28 antagonize the transcriptional activity of the RVE8/LNK1/LNK2 circadian complex
    Article Snippet: One μ g of total RNA was used as the template to synthesize cDNA using the iScript RT-PCR kit (Bio-Rad, Carlsbad, CA).

    Article Title: Effect of Pesticide Vinclozolin Toxicity Exposure on Cardiac Oxidative Stress and Myocardial Damage
    Article Snippet: An iScript RT-PCR kit (Bio-Rad, Hercules, CA, USA) was used to synthesize first-strand cDNA according to the manufacturer’s recommendations.

    Amplification:

    Article Title: Modulation of TLR4/NFκB Pathways in Autoimmune Myocarditis
    Article Snippet: An iScript RT-PCR kit (Bio-Rad, Hercules, CA, USA) was used to synthesize the first-strand cDNA [ ].

    Article Title: Enhanced Survival of Plasmodium -Infected Mosquitoes during Starvation
    Article Snippet: Total RNA was extracted from individual mosquito using the RNeasy Mini Kit (QIAGEN, CA). cDNA was synthesized using the iScript RT-PCR kit (Bio-Rad, CA).

    Article Title: Compositions and methods for dengue virus chimeric constructions in vaccines
    Article Snippet: The real time RT-PCR was performed with the iQ5 or CFX-95 system (BioRad), using a BioRad iScript RT-PCR (for probes) kit, in a 25-μl reaction containing 5 μl of viral RNA template, 0.4 uM of each primer, and 0.2 uM of the probe.

    Article Title: Identification and Characterization of Ixodes scapularis Antigens That Elicit Tick Immunity Using Yeast Surface Display
    Article Snippet: The same procedure was done with unfed ticks. cDNA was synthesized using the iScript RT-PCR kit (Biorad, CA) and analyzed by quantitative PCR for the expression of tick actin, p8, p19, p23 and p32 and genes using the primers listed in .

    Article Title: NOTCH2 Promotes Osteoclast Maturation and Metabolism and Modulates the Transcriptome Profile During Osteoclastogenesis.
    Article Snippet: Equal amounts of RNA were reverse-transcribed using the iScript RT-PCR kit (Bio-Rad, Hercules, CA) and amplified in the presence of specific primers (all from IDT) with the SsoAdvancedTM Universal SYBR Green Supermix (Bio-Rad) at 60oC for 40 cycles (Table S6).

    Article Title: COLD REGULATED GENE 27 and 28 antagonize the transcriptional activity of the RVE8/LNK1/LNK2 circadian complex
    Article Snippet: One μ g of total RNA was used as the template to synthesize cDNA using the iScript RT-PCR kit (Bio-Rad, Carlsbad, CA).

    Article Title: Effect of Pesticide Vinclozolin Toxicity Exposure on Cardiac Oxidative Stress and Myocardial Damage
    Article Snippet: An iScript RT-PCR kit (Bio-Rad, Hercules, CA, USA) was used to synthesize first-strand cDNA according to the manufacturer’s recommendations.

    SYBR Green Assay:

    Article Title: Modulation of TLR4/NFκB Pathways in Autoimmune Myocarditis
    Article Snippet: An iScript RT-PCR kit (Bio-Rad, Hercules, CA, USA) was used to synthesize the first-strand cDNA [ ].

    Article Title: Enhanced Survival of Plasmodium -Infected Mosquitoes during Starvation
    Article Snippet: Total RNA was extracted from individual mosquito using the RNeasy Mini Kit (QIAGEN, CA). cDNA was synthesized using the iScript RT-PCR kit (Bio-Rad, CA).

    Article Title: Compositions and methods for dengue virus chimeric constructions in vaccines
    Article Snippet: The real time RT-PCR was performed with the iQ5 or CFX-95 system (BioRad), using a BioRad iScript RT-PCR (for probes) kit, in a 25-μl reaction containing 5 μl of viral RNA template, 0.4 uM of each primer, and 0.2 uM of the probe.

    Article Title: Identification and Characterization of Ixodes scapularis Antigens That Elicit Tick Immunity Using Yeast Surface Display
    Article Snippet: The same procedure was done with unfed ticks. cDNA was synthesized using the iScript RT-PCR kit (Biorad, CA) and analyzed by quantitative PCR for the expression of tick actin, p8, p19, p23 and p32 and genes using the primers listed in .

    Article Title: NOTCH2 Promotes Osteoclast Maturation and Metabolism and Modulates the Transcriptome Profile During Osteoclastogenesis.
    Article Snippet: Equal amounts of RNA were reverse-transcribed using the iScript RT-PCR kit (Bio-Rad, Hercules, CA) and amplified in the presence of specific primers (all from IDT) with the SsoAdvancedTM Universal SYBR Green Supermix (Bio-Rad) at 60oC for 40 cycles (Table S6).

    Article Title: COLD REGULATED GENE 27 and 28 antagonize the transcriptional activity of the RVE8/LNK1/LNK2 circadian complex
    Article Snippet: One μ g of total RNA was used as the template to synthesize cDNA using the iScript RT-PCR kit (Bio-Rad, Carlsbad, CA).

    Article Title: Effect of Pesticide Vinclozolin Toxicity Exposure on Cardiac Oxidative Stress and Myocardial Damage
    Article Snippet: An iScript RT-PCR kit (Bio-Rad, Hercules, CA, USA) was used to synthesize first-strand cDNA according to the manufacturer’s recommendations.

    Western Blot:

    Article Title: Modulation of TLR4/NFκB Pathways in Autoimmune Myocarditis
    Article Snippet: An iScript RT-PCR kit (Bio-Rad, Hercules, CA, USA) was used to synthesize the first-strand cDNA [ ].

    Article Title: Enhanced Survival of Plasmodium -Infected Mosquitoes during Starvation
    Article Snippet: Total RNA was extracted from individual mosquito using the RNeasy Mini Kit (QIAGEN, CA). cDNA was synthesized using the iScript RT-PCR kit (Bio-Rad, CA).

    Article Title: Compositions and methods for dengue virus chimeric constructions in vaccines
    Article Snippet: The real time RT-PCR was performed with the iQ5 or CFX-95 system (BioRad), using a BioRad iScript RT-PCR (for probes) kit, in a 25-μl reaction containing 5 μl of viral RNA template, 0.4 uM of each primer, and 0.2 uM of the probe.

    Article Title: Identification and Characterization of Ixodes scapularis Antigens That Elicit Tick Immunity Using Yeast Surface Display
    Article Snippet: The same procedure was done with unfed ticks. cDNA was synthesized using the iScript RT-PCR kit (Biorad, CA) and analyzed by quantitative PCR for the expression of tick actin, p8, p19, p23 and p32 and genes using the primers listed in .

    Article Title: NOTCH2 Promotes Osteoclast Maturation and Metabolism and Modulates the Transcriptome Profile During Osteoclastogenesis.
    Article Snippet: Equal amounts of RNA were reverse-transcribed using the iScript RT-PCR kit (Bio-Rad, Hercules, CA) and amplified in the presence of specific primers (all from IDT) with the SsoAdvancedTM Universal SYBR Green Supermix (Bio-Rad) at 60oC for 40 cycles (Table S6).

    Article Title: COLD REGULATED GENE 27 and 28 antagonize the transcriptional activity of the RVE8/LNK1/LNK2 circadian complex
    Article Snippet: One μ g of total RNA was used as the template to synthesize cDNA using the iScript RT-PCR kit (Bio-Rad, Carlsbad, CA).

    Article Title: Effect of Pesticide Vinclozolin Toxicity Exposure on Cardiac Oxidative Stress and Myocardial Damage
    Article Snippet: An iScript RT-PCR kit (Bio-Rad, Hercules, CA, USA) was used to synthesize first-strand cDNA according to the manufacturer’s recommendations.

    TUNEL Assay:

    Article Title: Modulation of TLR4/NFκB Pathways in Autoimmune Myocarditis
    Article Snippet: An iScript RT-PCR kit (Bio-Rad, Hercules, CA, USA) was used to synthesize the first-strand cDNA [ ].

    Article Title: Enhanced Survival of Plasmodium -Infected Mosquitoes during Starvation
    Article Snippet: Total RNA was extracted from individual mosquito using the RNeasy Mini Kit (QIAGEN, CA). cDNA was synthesized using the iScript RT-PCR kit (Bio-Rad, CA).

    Article Title: Compositions and methods for dengue virus chimeric constructions in vaccines
    Article Snippet: The real time RT-PCR was performed with the iQ5 or CFX-95 system (BioRad), using a BioRad iScript RT-PCR (for probes) kit, in a 25-μl reaction containing 5 μl of viral RNA template, 0.4 uM of each primer, and 0.2 uM of the probe.

    Article Title: Identification and Characterization of Ixodes scapularis Antigens That Elicit Tick Immunity Using Yeast Surface Display
    Article Snippet: The same procedure was done with unfed ticks. cDNA was synthesized using the iScript RT-PCR kit (Biorad, CA) and analyzed by quantitative PCR for the expression of tick actin, p8, p19, p23 and p32 and genes using the primers listed in .

    Article Title: NOTCH2 Promotes Osteoclast Maturation and Metabolism and Modulates the Transcriptome Profile During Osteoclastogenesis.
    Article Snippet: Equal amounts of RNA were reverse-transcribed using the iScript RT-PCR kit (Bio-Rad, Hercules, CA) and amplified in the presence of specific primers (all from IDT) with the SsoAdvancedTM Universal SYBR Green Supermix (Bio-Rad) at 60oC for 40 cycles (Table S6).

    Article Title: COLD REGULATED GENE 27 and 28 antagonize the transcriptional activity of the RVE8/LNK1/LNK2 circadian complex
    Article Snippet: One μ g of total RNA was used as the template to synthesize cDNA using the iScript RT-PCR kit (Bio-Rad, Carlsbad, CA).

    Article Title: Effect of Pesticide Vinclozolin Toxicity Exposure on Cardiac Oxidative Stress and Myocardial Damage
    Article Snippet: An iScript RT-PCR kit (Bio-Rad, Hercules, CA, USA) was used to synthesize first-strand cDNA according to the manufacturer’s recommendations.

    Synthesized:

    Article Title: Modulation of TLR4/NFκB Pathways in Autoimmune Myocarditis
    Article Snippet: An iScript RT-PCR kit (Bio-Rad, Hercules, CA, USA) was used to synthesize the first-strand cDNA [ ].

    Article Title: Enhanced Survival of Plasmodium -Infected Mosquitoes during Starvation
    Article Snippet: Total RNA was extracted from individual mosquito using the RNeasy Mini Kit (QIAGEN, CA). cDNA was synthesized using the iScript RT-PCR kit (Bio-Rad, CA).

    Article Title: Compositions and methods for dengue virus chimeric constructions in vaccines
    Article Snippet: The real time RT-PCR was performed with the iQ5 or CFX-95 system (BioRad), using a BioRad iScript RT-PCR (for probes) kit, in a 25-μl reaction containing 5 μl of viral RNA template, 0.4 uM of each primer, and 0.2 uM of the probe.

    Article Title: Identification and Characterization of Ixodes scapularis Antigens That Elicit Tick Immunity Using Yeast Surface Display
    Article Snippet: The same procedure was done with unfed ticks. cDNA was synthesized using the iScript RT-PCR kit (Biorad, CA) and analyzed by quantitative PCR for the expression of tick actin, p8, p19, p23 and p32 and genes using the primers listed in .

    Article Title: NOTCH2 Promotes Osteoclast Maturation and Metabolism and Modulates the Transcriptome Profile During Osteoclastogenesis.
    Article Snippet: Equal amounts of RNA were reverse-transcribed using the iScript RT-PCR kit (Bio-Rad, Hercules, CA) and amplified in the presence of specific primers (all from IDT) with the SsoAdvancedTM Universal SYBR Green Supermix (Bio-Rad) at 60oC for 40 cycles (Table S6).

    Article Title: COLD REGULATED GENE 27 and 28 antagonize the transcriptional activity of the RVE8/LNK1/LNK2 circadian complex
    Article Snippet: One μ g of total RNA was used as the template to synthesize cDNA using the iScript RT-PCR kit (Bio-Rad, Carlsbad, CA).

    Article Title: Effect of Pesticide Vinclozolin Toxicity Exposure on Cardiac Oxidative Stress and Myocardial Damage
    Article Snippet: An iScript RT-PCR kit (Bio-Rad, Hercules, CA, USA) was used to synthesize first-strand cDNA according to the manufacturer’s recommendations.

    Real-time Polymerase Chain Reaction:

    Article Title: Modulation of TLR4/NFκB Pathways in Autoimmune Myocarditis
    Article Snippet: An iScript RT-PCR kit (Bio-Rad, Hercules, CA, USA) was used to synthesize the first-strand cDNA [ ].

    Article Title: Enhanced Survival of Plasmodium -Infected Mosquitoes during Starvation
    Article Snippet: Total RNA was extracted from individual mosquito using the RNeasy Mini Kit (QIAGEN, CA). cDNA was synthesized using the iScript RT-PCR kit (Bio-Rad, CA).

    Article Title: Compositions and methods for dengue virus chimeric constructions in vaccines
    Article Snippet: The real time RT-PCR was performed with the iQ5 or CFX-95 system (BioRad), using a BioRad iScript RT-PCR (for probes) kit, in a 25-μl reaction containing 5 μl of viral RNA template, 0.4 uM of each primer, and 0.2 uM of the probe.

    Article Title: Identification and Characterization of Ixodes scapularis Antigens That Elicit Tick Immunity Using Yeast Surface Display
    Article Snippet: The same procedure was done with unfed ticks. cDNA was synthesized using the iScript RT-PCR kit (Biorad, CA) and analyzed by quantitative PCR for the expression of tick actin, p8, p19, p23 and p32 and genes using the primers listed in .

    Article Title: NOTCH2 Promotes Osteoclast Maturation and Metabolism and Modulates the Transcriptome Profile During Osteoclastogenesis.
    Article Snippet: Equal amounts of RNA were reverse-transcribed using the iScript RT-PCR kit (Bio-Rad, Hercules, CA) and amplified in the presence of specific primers (all from IDT) with the SsoAdvancedTM Universal SYBR Green Supermix (Bio-Rad) at 60oC for 40 cycles (Table S6).

    Article Title: COLD REGULATED GENE 27 and 28 antagonize the transcriptional activity of the RVE8/LNK1/LNK2 circadian complex
    Article Snippet: One μ g of total RNA was used as the template to synthesize cDNA using the iScript RT-PCR kit (Bio-Rad, Carlsbad, CA).

    Article Title: Effect of Pesticide Vinclozolin Toxicity Exposure on Cardiac Oxidative Stress and Myocardial Damage
    Article Snippet: An iScript RT-PCR kit (Bio-Rad, Hercules, CA, USA) was used to synthesize first-strand cDNA according to the manufacturer’s recommendations.

    Expressing:

    Article Title: Modulation of TLR4/NFκB Pathways in Autoimmune Myocarditis
    Article Snippet: An iScript RT-PCR kit (Bio-Rad, Hercules, CA, USA) was used to synthesize the first-strand cDNA [ ].

    Article Title: Enhanced Survival of Plasmodium -Infected Mosquitoes during Starvation
    Article Snippet: Total RNA was extracted from individual mosquito using the RNeasy Mini Kit (QIAGEN, CA). cDNA was synthesized using the iScript RT-PCR kit (Bio-Rad, CA).

    Article Title: Compositions and methods for dengue virus chimeric constructions in vaccines
    Article Snippet: The real time RT-PCR was performed with the iQ5 or CFX-95 system (BioRad), using a BioRad iScript RT-PCR (for probes) kit, in a 25-μl reaction containing 5 μl of viral RNA template, 0.4 uM of each primer, and 0.2 uM of the probe.

    Article Title: Identification and Characterization of Ixodes scapularis Antigens That Elicit Tick Immunity Using Yeast Surface Display
    Article Snippet: The same procedure was done with unfed ticks. cDNA was synthesized using the iScript RT-PCR kit (Biorad, CA) and analyzed by quantitative PCR for the expression of tick actin, p8, p19, p23 and p32 and genes using the primers listed in .

    Article Title: NOTCH2 Promotes Osteoclast Maturation and Metabolism and Modulates the Transcriptome Profile During Osteoclastogenesis.
    Article Snippet: Equal amounts of RNA were reverse-transcribed using the iScript RT-PCR kit (Bio-Rad, Hercules, CA) and amplified in the presence of specific primers (all from IDT) with the SsoAdvancedTM Universal SYBR Green Supermix (Bio-Rad) at 60oC for 40 cycles (Table S6).

    Article Title: COLD REGULATED GENE 27 and 28 antagonize the transcriptional activity of the RVE8/LNK1/LNK2 circadian complex
    Article Snippet: One μ g of total RNA was used as the template to synthesize cDNA using the iScript RT-PCR kit (Bio-Rad, Carlsbad, CA).

    Article Title: Effect of Pesticide Vinclozolin Toxicity Exposure on Cardiac Oxidative Stress and Myocardial Damage
    Article Snippet: An iScript RT-PCR kit (Bio-Rad, Hercules, CA, USA) was used to synthesize first-strand cDNA according to the manufacturer’s recommendations.



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